Role of Lanc like G protein-coupled receptor-2with BOP and BTB/POZ in Stress Tolerance and High Yielding trait of Pigeonpea

Abstract

Pigeonpea is drought resilient crop; relatively more drought tolerant than other legume crops. Through detailed evaluation and multi-location trials of cross derivatives, we identified 65 better performing pigeonpea lines. Among these lines, high yielding and stress-tolerant accessions were identified. From our earlier MYB network and flowering genes networks, we could identify tightly linked co-expressing genes for yield traits. Semi-quantitative expression analyses showed that the defending type drought stress tolerance contributing LAN C like protein GCL-2 is expressed in providing disease resistance and myb linked BTB/POZ genes contribute for high yielding of pigeonpea. BOP is a member of BTB group of plant protein. We found differential upregulation of these genes in drought-tolerant high yielding pigeonpea lines earlier reported by our team. Whereas in another report we explained the myb linked expression of BTB/POZ genes. These genes
selected from our earlier network analyses were identified, PCR amplified, sequenced and structure validated for its functional domain. Using the gene sequence, we predicted and validated the protein structure of Lan C. The current study extends our earlier findings that these genes are directly taking part in stress tolerance
and high yielding traits.

Keywords: Pigeonpea, stress, protein, LanC, gene